Lisandro Abadie was born in Buenos Aires, where he began his vocal studies. He continued his training in Switzerland at the Schola Cantorum Basiliensis and the Lucerne School of Music and was awarded the Edwin Fischer Memorial Prize in 2006.

He has performed under the direction of renowned conductors such as William Christie, Laurence Cummings, Václav Luks, Francesco Corti, Rubén Dubrovsky, Tōnu Kaljuste, Andreas Reize, Jordi Savall, Paul Agnew, Skip Sempé, Vincent Dumestre and Geoffroy Jourdain.

Abadie regularly collaborates with leading ensembles including Les Arts Florissants, Collegium 1704, Orchestra of the Age of Enlightenment, Les Talens Lyriques, Le Poème Harmonique, La Tempête, Ensemble Inégal, La Risonanza and a nocte temporis. He has also worked closely with the lutenist Mónica Pustilnik, the pianist and composer Paul Suits, and the stage director Benjamin Lazar.

His recent stage appearances include Charpentier's Médée’ Rossini's La Cenerentola, Vivaldi's Arsilda, Monteverdi's operatic trilogy, as well as  Alcione, Phaëton, L'Europe Galante and Les Fées du Rhin. His discography includes recordings of Handel's Siroe and Music for Queen Caroline, Lully's Phaeton and Cadmus et Hermione, Pasquini's L'ombra di Solimano (bass cantatas), and Monteverdi's madrigals, Vespro and L'incoronazione di Poppea. His work as a soloist is documented in around sixty CD and DVD recordings, as well as numerous television and online broadcasts.

Alongside his performing career, Abadie engages in research and writing on the history of singing and vocal technique. He is active as a teacher and regularly gives masterclasses, including with William Christie in Thiré, at the Balthasar Neumann Academy in Fontainebleau and in Havana, as well as at conservatoires in France and Germany. He is also teaches at the Monteverdi Madrigal Week in Venice.

In 2024, he made his debut at the Opéra de Paris and the Teatro Real in Madrid in Charpentier's Médée under the direction of William Christie. He has taught at the Schola Cantorum Basiliensis since 2019 and at Mozarteum University Salzburg since 2024.